Journal: Journal of Veterinary Internal Medicine

Article Title: Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device

doi: 10.1111/jvim.16105

Figure Lengend Snippet: Fluorescence emission from the microfluidic device following 40 minutes of S. equi DNA LAMP amplification. There is equal fluorescence at 40 minutes whether LAMP detects 1 CFU or 100 CFU. CFU, colony forming unit; LAMP, loop‐mediated isothermal nucleic acid amplification

Article Snippet: Logistic regression power analysis determined a sample size of 60 GPL specimens were enough for the study to demonstrate a difference between the 3 DNA amplification methods (power of 80% and α ≤ .05) (STATA 14.0).

Techniques: Fluorescence, Amplification

Journal: Journal of Veterinary Internal Medicine

Article Title: Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device

doi: 10.1111/jvim.16105

Figure Lengend Snippet: (A) Fluorescent by‐product of LAMP distinguished a positive vs negative result of S. equi DNA detection on dry storage microfluidic device (B) as seen via a smartphone screen. LAMP, loop‐mediated isothermal nucleic acid amplification

Article Snippet: Logistic regression power analysis determined a sample size of 60 GPL specimens were enough for the study to demonstrate a difference between the 3 DNA amplification methods (power of 80% and α ≤ .05) (STATA 14.0).

Techniques: Amplification

Journal:

Article Title: Matrilysin [MMP-7] Expression Selects for Cells with Reduced Sensitivity to Apoptosis 1

doi:

Figure Lengend Snippet: Fas and FasL are expressed and the Fas pathway is functional in NMuMG and HBL00 cells. (A) Western blotting of total cell lysates from HBL100 and NMuMG cells for Fas and FasL, using the M20 anti-Fas and C178 anti-FasL antibodies, respectively. (B) Flow cytometric analysis of cell-surface expression of Fas and FasL using the clone 13 anti-Fas and clone 33 anti-FasL antibodies. The solid black peak in each graph indicates the background staining observed with a control IgG. Similar traces were obtained when the M20 anti-Fas and C178 anti-FasL antibodies were used (not shown). (C) Immunoprecipitation of biotinylated surface-associated FasL. For each cell line, lysates from biotinylated cells were subjected to the immunoprecipitation procedure in the absence (No I.P.) or presence (Fas-Fc I.P.) of Fas-Fc protein. A band of approximately 40 kDa was specifically detected in the I.P. lane for each cell line. (D) Genomic DNA was isolated from NMuMG or HBL100 cells that had been treated with the Fas-activating antibodies Jo-2 and CH11, respectively (+) or isotype control (-) and was analyzed by agarose gel electrophoresis. Cycloheximide at a concentration of 1 µg/ml was included with the NMuMG cells. The characteristic DNA laddering pattern associated with apoptosis can be clearly seen in the Fas antibody-treated lanes.

Article Snippet: The blots were washed and incubated with biotinylated anti-species secondary antibodies (Vector Laboratories, Burlingame, CA).

Techniques: Functional Assay, Western Blot, Expressing, Staining, Immunoprecipitation, Isolation, Agarose Gel Electrophoresis, Concentration Assay, DNA Laddering